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N4754

Sigma-Aldrich

Nigrosin water soluble

Powder

Sinónimos:

Acid black 2

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About This Item

Número de CAS:
8005-03-6
Colour Index Number:
50420
MDL number:
MFCD00044681
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Nigrosin water soluble, For use as a biological stain

Quality Level

200

form

powder

solubility

water: 1 mg/mL, turbid

εmax

~250 at 575 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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General description

Nigrosin is an acidic dye used as a negative stain for visualization of the capsule in bacteriological material.

Application

Nigrosin has the following applications:
  • to stain live sperm
  • as a viable cell staining agent especially in stem cell biology
  • as a negative stain in place of India ink for spirochetes, bacteria, protozoa, and fungi
  • in Dorner′s spore stain.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCF8336
BCBX1760
BCBR0628V
BCBN9273V
BCBJ9097V

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Visite la Librería de documentos

Graff's Textbook of Routine Urinalysis and Body Fluids (2010)
Experiments in Microbiology, Plant Pathology and Biotechnology (2003)
Broadband UV-Vis optical property measurement in layered turbid media.
Wang Q
SPIE Medical Imaging. International Society for Optics and Photonics, 2014 (2011)
Quanzeng Wang et al.
Biomedical optics express, 3(6), 1226-1240 (2012-06-29)
The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of
L Björndahl et al.
Human reproduction (Oxford, England), 18(4), 813-816 (2003-03-28)
The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. The technique was
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