2GFHSNTMGKB
Roche
KAPA2G Fast HotStart PCR Kit
with dNTPs & Mg-free buffers
Sinónimos:
PCR, hotstart PCR
About This Item
Productos recomendados
Quality Level
usage
sufficient for 1000 reactions
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Fast PCR
dNTPs included
hotstart
packaging
pkg of 250 U (KK5512)
pkg of 500 U (KK5510)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
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General description
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
Application
- Routine PCR
- Fast PCR
- Genotyping
Biochem/physiol Actions
Features and Benefits
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes:
- KAPA2G Fast HotStart PCR Kits with dNTPs & Mg-free buffers contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields,specificity and sensitivity, facilitating efficient primerannealing across a wide range of primer lengths,GC contents, and melting temperatures.
- Use 0.5 U KAPA2G Fast HotStart DNA Polymerase per 25 μL reaction, or less for smaller volumes.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- The buffer is available with and without MgCl2 KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X, while KAPA2G Buffer M is Mg-free. Aside from MgCl2 content, these two buffers are identical.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Legal Information
Solo componentes del kit
- KAPA2G Fast Standard or HotStart® DNA Polymerase 5 U/μL
- 5X KAPA2G Buffer M (Mg-free; available with select HotStart kits)
- MgCl2 25 mM
- dNTP Mix (optional) 10 mM each dNTP
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
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