MABC1564

Anti-Pin1 Antibody, clone 5B4.1

clone 5B4.1, from mouse

• Sinónimos: Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC: 5.2.1.8, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5B4.1, monoclonal
• species reactivity: human, rat
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_006212
• UniProt accession no.: Q13526
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... PIN1(5300)

Descripción

General description

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (UniProt: Q13526; also known as EC: 5.2.1.8, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1) is encoded by the PIN1 gene (Gene ID: 5300) in human. PPIase binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. By inducing conformational changes in a subset of phosphorylated proteins, it acts as a molecular switch in multiple cellular processes. PPIase is mainly localized in the nucleus, but phosphorylation at Serine 71 by death-associated protein kinase 1 (DAPK1) results in inhibition of its nuclear localization and its catalytic activity. It contains a WW domain (aa 5-39) and a peptidylprolyl isomerase C (PpiC) domain (aa 52-163). WW domain is shown to be essential for the interaction with STIL and KIF20B. PPIase displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. PPIase is reported to regulate mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. It is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53, NF-kB, c-Jun, cyclin D1, and beta-catenin.

Specificity

Clone 5B4.1 detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) in human and rat cells. It targets an epitope within 92 amino acids from the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the C-terminal half of human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1.

Application

Anti-Pin1, clone 5B4.1, Cat. No. MABC1564, is a mouse monoclonal antibody that detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pin1 in human cerebral cortex tissue sections.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pin1 in 10 µg of PC-12 cell lysate.

Target description

~18 kDa observed; 18.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash