DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. Markers and ladders stain well with ethidium bromide and other common nucleic acid stains for visualization after gel electrophoresis.
We offer a variety of markers for nucleic acid size determination of samples separated by various gel electrophoresis methods.
RNA electrophoresis requires markers prepared from RNA. The Transcript RNA Marker contains nine RNA transcripts. The ladder is diluted in gel loading buffer and then loaded in a single lane on an agarose gel. Markers are suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis. The Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization. The Transcript RNA Marker is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA and contains nine defined RNA transcripts. For optimal resolution, the recommended agarose gel concentration is 1.0%.