Many polymerase chain reaction (PCR) applications require longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase. Conventional Taq DNA polymerase lacks proofreading capability, resulting in a relatively high error rate. Taq polymerase also tends to disassociate from the template and cease elongation at sites of misincorporated nucleotides. Cloning and expression experiments, cDNA analysis and array work can be improved by using long and accurate amplification over conventional PCR.
Long and accurate (LA) amplification is achieved by combining a thermostable polymerase with a second polymerase exhibiting a 3'—>5' exonuclease activity. The exonuclease, or proofreading, activity repairs terminal misincorporations. This repair allows for greater read lengths and increased fidelity.
We offer products to achieve successful long and accurate PCR. Our AccuTaq™ LA enzyme and blends provide increased yield, higher processivity for greater lengths, and proofreading activity for increased fidelity over other long and accurate enzymes.
AccuTaq™ LA DNA Polymerase (Product No. D8045) and REDAccuTaq™ LA DNA Polymerase (Product No. D4812) are blends of our quality Taq DNA polymerase with a 3'—>5' proofreading exonuclease. This mix provides increased yield and 6.5X greater fidelity over standard Taq DNA polymerase.
Combining an antibody-based hot start mechanism with our long-and-accurate enzymes results in increased specificity and sensitivity to our high yield, high fidelity products. JumpStart™ AccuTaq™ (Product No. D5809) and JumpStart™ REDAccuTaq™ LA DNA Polymerase (Product No. D1313) are ideal for high fidelity cloning applications or high yield array work.