Our Ascentis® Express F5 column brings together the highly efficient, robust Fused-Core® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.
The pentafluorophenylpropyl (PFP) stationary phase of the Ascentis® Express F5 column, based on Fused-Core® particle technology, provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express F5 columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 phases.
Comparision of C18 and F5 for the Separation of Closely Related Steroids
Ascentis® Express F5 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. 3 different particle sizes are available for your specific needs:
2.0 µm particles
Best Fused-Core UHPLC Column
An optimized solution for high-throughput small molecule analysis
2.7 µm particles
Fast HPLC on ANY System
A practical solution that delivers UHPLC performance from any HPLC
5.0 µm particles
The Lab Work-Horse Column
True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns