Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Anita Piper

Life Science Application Centre (GAC), Darmstadt, Germany

Introduction

Neonicotinoids are water soluble insecticides. They are used for crop protection and can migrate from treated soil into plant tissue and flower nectar. Neonicotinoids have a negative effect on brain cells of honey bees, and colony collapse can occur. [Clothianidin, Imidacloprid and Thiamethoxam are banned in Germany, (EU) No. 485/2013].

This application focuses on HPLC testing of neonicotinoids in honey. The limit of detection (LOD) and limit of quantitation (LOQ) for neonicotinoids is better than 30 µM and 90 µM, respectively, when using HPLC combined with UV detection.

Determination of Neonicotinoids in Honey

Chromatographic Data - blank run

Chromatographic Data - blank run
Chromatographic Data - blank run

Chromatographic Data - Standard Solution

Standard Solution (200 µM @ 254 nm)

Standard Solution (200 µM @ 254 nm)

Standard Solution (200 µM @ 270 nm)

Standard Solution (200 µM @ 270 nm)

Chromatographic Data - Sample Solution spiked

Sample Solution Spiked @ 254 nm

Sample Solution Spiked @ 254 nm

Sample Solution @ 270 nm

 Sample Solution @ 270 nm

Chromatographic Data - Sample Solution spiked

Sample Solution Spiked @ 254 nm

Sample Solution @ 254 nm

Sample Solution Spiked @ 270 nm

Sample Solution Spiked @ 270 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @254 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @270 nm

2. Standard repeatability honey (spiked with 5 µL of a 200 µM standard solution) in mAU*min

3. LOD & LOQ

4. Linearity (area mAU*min)

Dinutefuran @270 nm

Dinutefuran

Nitenpyram @270 nm

Nitenpyram

Thiamethoxam @254 nm

Thiamethoxam

Imidacloprid @270 nm

Imidacloprid

Clothianidin @270 nm

Clothianidin

Acetamiprid @254 nm

Acetamiprid

Thiacloprid @254 nm

Thiacloprid
Materials List
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