We are working hand in hand with public health authorities and customers to support the fight against novel coronavirus (SARS-CoV-2). In response to the community’s need for SARS-CoV-2-specific reagents and protocols, our scientists have designed qPCR assays for SARS-CoV-2 target detection (Table 1). Research Use Only. Not for Use in Diagnostic Procedures.
2019-nCoV shares 95% homology with SARS viruses, creating a high risk of cross-reactivity. Please keep the following notes in mind while designing your experiments using Table 1:
To better differentiate between SARS viruses and SARS-CoV-2, we designed the following SARS positive control assay (Table 2). Please keep the following notes in mind while designing your experiments using Table 2:
Our KiCqStart® Dual-Labeled Probes are labeled with our own license and royalty-free dark quencher, Onyx Quencher (OQ). OQ is also available with custom probe orders.
We have a commercial license to supply probes labeled with Black Hole Quencher® (BHQ®) & BlackBerry® Quencher (BBQ®) to detect SARS-CoV-2.
To request a quote for custom primers and probes, please email us.
The SARS-CoV-2 virus has a positive-sense ssRNA genome. We offer in vitro transcribed ssRNA against regions of this genome, which can serve as positive controls. We can synthesize custom sequences from 150 to 420 nucleotides (to ensure successful manufacturing, please avoid sequences with inverted repeats and repetitions). For Research Use Only. Not for Use In Diagnostic Procedures.
To request a quote or additional help, please email us.
We are committed to providing researchers with the reagents and technical expertise for timely support of the SARS-CoV-2 outbreak. Explore our offering of world-class RT-PCR, RT-qPCR and supporting reagents that are suitable for SARS-CoV-2 vaccine research.
Utilizing hot start one-step RT-PCR technology, Transcriptor One-Step RT-PCR Kit includes a mixture of enzymes, including Taq DNA polymerase and a proofreading polymerase, to ensure sensitive and robust reverse transcription. Except for template and primers, Transcriptor One-Step RT-PCR Kit provides all the components required for one-step RT-PCR.
KAPA PROBE FAST One-Step is designed for high-throughput, fast-cycling, one-step RNA quantification. KAPA PROBE FAST One-Step is suitable for use with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
Incorporating a mixture of three enzymes, Titan™ One Tube RT-PCR System utilizes reverse transcriptase AMV for first-strand cDNA synthesis and the Expand™ High Fidelity enzyme blend, consisting of Taq DNA polymerase and a polymerase with a proofreading activity, for amplification of cDNA by PCR.
In addition to containing Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) for first-strand cDNA synthesis, the Quantitative RT-PCR ReadyMix™ is conveniently blended with JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, stabilizers, and ships as a 2x concentrate.
Suitable for all dual-labeled probe chemistries, KiCqStart® One-Step Probe RT-qPCR ReadyMix™ comes with all required components for RT-qPCR, except for RNA template and probe. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is available with or without ROX™ reference dye.
Providing a highly sensitive method for the quantitative analysis of gene expression, this kit combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix™.
Our MISSION® esiRNA COVID-19 panel (51 genes with 2 controls) provides RNAi researchers with a proven, cost-effective, and simple way to perform post-transcriptional silencing of putative genes involved in the intracellular life cycle of SARS-CoV-2. Included in the panel are genes that were found to be involved with the infection caused by other coronaviruses, such as SARS-CoV and MERS. This panel is available as a customizable esiFLEX 96-well array at quantities up to 5.0 μg, which is sufficient for screening common human cell lines. For Research Use Only. Not for Use In Diagnostic Procedures.
Included with the panels is the following:
To request a quote or additional help, please email us.
We will continue to monitor the situation and work closely with customers to support response efforts to this WHO global emergency. Please pass along our primer and probe sets for qPCR detection of SARS-CoV-2 and biochemicals for Vaccine and Diagnostics research (Download PDF).
Contact us for expedited support in SARS-CoV-2 vaccine development.