1 base (K3PO4, 1.5 M solution in degassed water)
4 degassed solvents (DMAc, Toluene, n-Butanol, and THF)
6 pre-weighed catalysts (x4)
Additional (not included) items needed:
Figure 1.Dose instructions for DMAc, Toluene, n-butanol, THF, and K3PO4
Note: This recipe makes 50 mL which is enough stock solution for all four screening sets in the KitAlysis Suzuki-Miyaura Cross-Coupling Reaction Screening Kit. The amount of internal standard is 10 mol% per reaction. So a big product peak to small internal standard indicates a good reaction. Integrate to compare reactions against one another (product/internal standard).
Sealing the plate properly is critical to success. The key is light, even pressure to the lid of the block to keep the cover flat while sealing.
Figure 2.Screw pattern for 24-well reaction plates
Use a small oil bath or other metal block (for best results) in the back of the hot plate and place the probe in there. Place reaction block as close to the center of the hot plate as possible for more even stirring.
Due to narrow opening of pipette tips, slurry additions will result in blockages. To aid in uniform dosing, simply snip off the end of the tip at the first marker (~10 mm). To ensure an evenly dispersed aliquot, it is critical that the mixture is stirring well while you draw aliquots for dosing into the corresponding reaction vial.
Figure 3.Snip approx. 10 mm from end of pipette