Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liquid nitrogen in the cryovials to burst and cause injury.
Figure 1.Rat Brain Microvascular Endothelial Cells (RBMVEC)
Prepare as in Step III A.
Trypsinize Cells at Room Temperature. Do Not Warm Any Reagents to 37 °C.