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  • SelexGLM differentiates androgen and glucocorticoid receptor DNA-binding preference over an extended binding site.

SelexGLM differentiates androgen and glucocorticoid receptor DNA-binding preference over an extended binding site.

Genome research (2017-12-03)
Liyang Zhang, Gabriella D Martini, H Tomas Rube, Judith F Kribelbauer, Chaitanya Rastogi, Vincent D FitzPatrick, Jon C Houtman, Harmen J Bussemaker, Miles A Pufall
ABSTRACT

The DNA-binding interfaces of the androgen (AR) and glucocorticoid (GR) receptors are virtually identical, yet these transcription factors share only about a third of their genomic binding sites and regulate similarly distinct sets of target genes. To address this paradox, we determined the intrinsic specificities of the AR and GR DNA-binding domains using a refined version of SELEX-seq. We developed an algorithm,

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Sigma-Aldrich
Pipette filler for calibrated microcapillary pipettes, dispenses up to 100 μL