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Starch-enhanced degradation of HMW PAHs by Fusarium sp. in an aged polluted soil from a coal mining area.

Chemosphere (2017-02-16)
Ou-Ya Zhao, Xue-Na Zhang, Sheng-Dong Feng, Li-Xiu Zhang, Wei Shi, Zhi-Xin Yang, Miao-Miao Chena, Xue-Dan Fanga
ABSTRACT

The present study used strain ZH-H2 (Fusarium sp.) isolated by our group as the PAH-degrading strain and 5-6-rings PAHs as degradation objects. The soil incubation experiment was carried out to investigate the starch-enhanced degradation effects of HMW PAHs by Fusarium sp. in an Aged Polluted Soil from a Coal Mining Area. The results showed that the removal rates of BaP, InP and BghiP increased with increasing inoculation rate of ZH-H2 in the unsterile aged polluted soil of coal mining area, with the exception of BbF degradation which increased in the H2 treatment and then decreased. Different addition dosage of starch apparently resulted in degradation of 4 PAHs in soil, with removal rates of 14.47% for BaP, 23.83% for DbA, 30.77% for BghiP and 31.00% for InP obtained with treatment D2, respectively higher than in treatment D1. So starch addition apparently enhanced the degradation of the 4 PAHs, especially InP and BghiP, by native microbes in the aged HMW PAH-polluted soil. By adding starch to these aged polluted soils with inoculated strain ZH-H2, HMW-PAHs degradation was further improved and addition of 0.5 g kg

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Bromo-2-fluorobiphenyl, 99%
Supelco
Perylene-d12 solution, certified reference material, 2000 μg/mL in dichloromethane
Supelco
p-Terphenyl-d14 solution, certified reference material, 2000 μg/mL in dichloromethane