MilliporeSigma
  • Home
  • Search Results
  • Poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing scaffolds and their integration with osteoblasts as a model for bone tissue engineering.

Poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing scaffolds and their integration with osteoblasts as a model for bone tissue engineering.

Journal of biomaterials applications (2015-01-17)
Sai Zhang, Molamma P Prabhakaran, Xiaohong Qin, Seeram Ramakrishna
ABSTRACT

Nano/micro engineered polymeric materials offer expansive scope of biomimetic scaffolds for bone tissue engineering especially those involving electrospun biodegradable nanofibers incorporated with inorganic nanoparticles, thus mimicking the extracellular matrix of bone both structurally and chemically. For the first time, poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing natural poly-(α, β)-DL-aspartic acid and inorganic hydroxyapatite nanofibers were fabricated using poly-3-hydroxybutyrate-co-3-hydroxyvalerate: poly-(α, β)-DL-aspartic acid at a ratio of 80:20 (w/w) added with 1% (w/v) of hydroxyapatite, by the process of electrospinning. The surface morphology, chemical, and mechanical properties of electrospun poly-3-hydroxybutyrate-co-3-hydroxyvalerate, poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid, and poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid/hydroxyapatite nanofibers were characterized by using field emission scanning electron microscope, Fourier transform infrared spectroscopy, and tensile tester, respectively. Human fetal osteoblasts were cultured on different nanofibrous scaffolds for evaluating the cell proliferation, alkaline phosphatase activity, and mineralization. Cells on poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid/hydroxyapatite scaffolds demonstrated higher proliferation (30.10%) and mineral deposition (37.60%) than the cells grown on pure poly-3-hydroxybutyrate-co-3-hydroxyvalerate scaffolds. Obtained results highlight the synergistic effect of poly-3-hydroxybutyrate-co-3-hydroxyvalerate, poly-(α, β)-DL-aspartic acid, and hydroxyapatite towards the enhancement of the osteoinductivity and osteoconductivity of human fetal osteoblasts, demonstrating the appropriate physicochemical and biological properties of poly-3-hydroxybutyrate-co-3-hydroxyvalerate/poly-(α, β)-DL-aspartic acid/hydroxyapatite nanofibers to function as a substrate for bone tissue regeneration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
Sigma-Aldrich
Activated Charcoal Norit®, Norit® CA1, wood, chemically activated, powder
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
Sigma-Aldrich
Glutaraldehyde solution, 50 wt. % in H2O
Sigma-Aldrich
Glutaric dialdehyde solution, 50 wt. % in H2O, FCC
Sigma-Aldrich
1,1,1,3,3,3-Hexafluoro-2-propanol, ≥99%
Supelco
1,1,1,3,3,3-Hexafluoro-2-propanol, for GC derivatization, LiChropur, ≥99.8%
Sigma-Aldrich
Cetylpyridinium chloride, meets USP testing specifications
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Supelco
Activated Charcoal Norit®, Norit® RBAA-3, rod
Sigma-Aldrich
Carbon, mesoporous
Sigma-Aldrich
Carbon, mesoporous, average pore diameter 100±10 Å (typical), >99.95% trace metals basis
Sigma-Aldrich
Carbon, mesoporous, nanopowder, graphitized, <500 nm particle size (DLS), >99.95% trace metals basis
Sigma-Aldrich
Carbon, mesoporous, nanopowder, <500 nm particle size (DLS), >99.95% trace metals basis
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
Cetylpyridinium chloride, European Pharmacopoeia (EP) Reference Standard
USP
Cetylpyridinium chloride, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Ethylenediaminetetraacetic acid solution, BioUltra, for molecular biology, pH 8.0, ~0.5 M in H2O
Sigma-Aldrich
Carbon nanofibers, graphitized (iron-free), composed of conical platelets, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Hexamethyldisilazane, reagent grade, ≥99%