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Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

Cell biochemistry and biophysics (2014-08-16)
Paweł Mochalski, Markus Theurl, Andreas Sponring, Karl Unterkofler, Rudolf Kirchmair, Anton Amann
ABSTRACT

Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehydes (2-methyl 2-propenal, 2-methyl propanal, 2-methyl butanal, 3-methyl butanal, n-hexanal, benzaldehyde, n-octanal and n-nonanal) and n-butyl acetate. Further eight compounds (ethyl acetate, ethyl propanoate, ethyl butyrate, 3-heptanone, 2-octanone, 2-nonanone, 2-methyl-5-(methylthio)-furan and toluene) were found to be emitted by the cells under study. Possible metabolic pathways leading to the uptake and release of these compounds by HUVEC are proposed and discussed. The uptake of aldehydes by endothelial cells questions the reliability of species from this chemical class as breath or blood markers of disease processes in human organism. The analysis of volatiles released or emitted by cell lines is shown to have a potential for the identification and assessment of enzymes activities and expression.

MATERIALS
Product Number
Brand
Product Description

Supelco
Nonanal, analytical standard
Sigma-Aldrich
Hexanal, ≥97%, FCC, FG
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Hexanal, natural, ≥95%, FG
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Ethyl acetate, ACS reagent, ≥99.5%
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2-Nonanone, natural, ≥97%, FCC, FG
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2-Nonanone, ≥99%, FCC, FG
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Toluene, anhydrous, 99.8%
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Ethyl acetate, anhydrous, 99.8%
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2-Nonanone, ≥99%
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2-Octanone, ≥98%, FG
Supelco
2-Octanone, analytical standard
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Methacrolein, 95%
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Hexanal, 98%
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Ethyl acetate, HPLC grade, 99.8%
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Nonanal, 95%
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Isobutyraldehyde, ≥99%
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Isobutyraldehyde, redistilled, ≥99.5%
Supelco
Toluene, analytical standard
Supelco
2-Nonanone, analytical standard
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Isobutyraldehyde, natural, 96%, FG
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Isobutyraldehyde, ≥98%, FG
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2-Octanone, reagent grade, 98%
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Ethyl acetate, ReagentPlus®, ≥99.8%
Supelco
Ethyl acetate, analytical standard
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Nonanal, ≥95%, FCC
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Isobutyraldehyde, 98%
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Isobutyraldehyde, dry, 98%
Supelco
Toluene, HPLC grade, 99.8%
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Ethyl acetate, natural, ≥99%, FCC, FG
Sigma-Aldrich
Ethyl acetate, ≥99%, FCC, FG