• Home
  • Search Results
  • Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

Cell biochemistry and biophysics (2014-08-16)
Paweł Mochalski, Markus Theurl, Andreas Sponring, Karl Unterkofler, Rudolf Kirchmair, Anton Amann
ABSTRACT

Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehydes (2-methyl 2-propenal, 2-methyl propanal, 2-methyl butanal, 3-methyl butanal, n-hexanal, benzaldehyde, n-octanal and n-nonanal) and n-butyl acetate. Further eight compounds (ethyl acetate, ethyl propanoate, ethyl butyrate, 3-heptanone, 2-octanone, 2-nonanone, 2-methyl-5-(methylthio)-furan and toluene) were found to be emitted by the cells under study. Possible metabolic pathways leading to the uptake and release of these compounds by HUVEC are proposed and discussed. The uptake of aldehydes by endothelial cells questions the reliability of species from this chemical class as breath or blood markers of disease processes in human organism. The analysis of volatiles released or emitted by cell lines is shown to have a potential for the identification and assessment of enzymes activities and expression.

MATERIALS
Product Number
Brand
Product Description

Supelco
Butyl acetate, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
3-Heptanone, analytical standard
Supelco
Benzaldehyde, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Isovaleraldehyde, analytical standard
Supelco
Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Butyl acetate, suitable for HPLC, 99.7%
Sigma-Aldrich
Butyl acetate, puriss. p.a., ACS reagent
Sigma-Aldrich
Butyl acetate, ACS reagent, ≥99.5%
Sigma-Aldrich
Butyl acetate, ReagentPlus®, 99.5%
Sigma-Aldrich
3-Heptanone, 98%
Supelco
2-Methylbutyraldehyde, analytical standard
Benzaldehyde, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Ethyl propionate, natural, ≥97%, FCC, FG
Sigma-Aldrich
2-Methylbutyraldehyde, ≥95%, FG
Sigma-Aldrich
Ethyl propionate, 99%
Sigma-Aldrich
Butyl acetate, ≥99%, FCC, FG
Sigma-Aldrich
Butyl acetate, anhydrous, ≥99%
Sigma-Aldrich
Benzaldehyde, purified by redistillation, ≥99.5%
Sigma-Aldrich
Benzaldehyde, natural, FCC, FG
Sigma-Aldrich
Octanal, 99%
Sigma-Aldrich
Ethyl butyrate, 99%
Sigma-Aldrich
2-Methylbutyraldehyde, 95%
Sigma-Aldrich
Ethyl propionate, ≥97%, FCC, FG
Supelco
Octanal, analytical standard
Sigma-Aldrich
Ethyl butyrate, ≥98%, FCC, FG
Sigma-Aldrich
Octanal, ≥95%, FCC, FG
Sigma-Aldrich
Octanal, natural, ≥95%, FCC, FG
Sigma-Aldrich
Isovaleraldehyde, natural, ≥95%, FG
Sigma-Aldrich
Benzaldehyde, ReagentPlus®, ≥99%
Sigma-Aldrich
Isovaleraldehyde, ≥97%, FG