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Advanced oxidation protein products as novel mediators of inflammation and monocyte activation in chronic renal failure.

Journal of immunology (Baltimore, Md. : 1950) (1998-09-02)
V Witko-Sarsat, M Friedlander, T Nguyen Khoa, C Capeillère-Blandin, A T Nguyen, S Canteloup, J M Dayer, P Jungers, T Drüeke, B Descamps-Latscha
ABSTRACT

We previously demonstrated the presence of advanced oxidation protein products (AOPP), a novel marker of oxidative stress in the plasma of uremic patients receiving maintenance dialysis. The present study in a cohort of 162 uremic patients showed that plasma concentrations of AOPP increased with progression of chronic renal failure and were closely related to advanced glycation end products (AGE)-pentosidine (r = 0.52, p < 0.001), taken as a marker of AGE. In vivo, the relevance of AOPP and AGE-pentosidine in monocyte-mediated inflammatory syndrome associated with uremia was evidenced by close correlations between AOPP or AGE-pentosidine and monocyte activation markers, including neopterin, IL-1R antagonist, TNF-alpha, and TNF soluble receptors (TNF-sR55 and TNF-sR75). To determine the mechanisms by which AOPP and AGE could be directly involved in monocyte activation, AOPP-human serum albumin (HSA) and AGE-HSA were produced in vitro by treating HSA with oxidants or glucose, respectively. Spectroscopic analysis confirmed that AOPP-HSA contains carbonyls and dityrosine. Both AOPP-HSA and AGE-HSA, but not purified dityrosine, were capable of triggering the oxidative burst of human monocytes in cultures. The AOPP-HSA-induced respiratory burst was dependent on the chlorinated nature of the oxidant and on the molar ratio HSA/HOCI. Collectively, these data first demonstrate that AOPP act as a mediator of oxidative stress and monocyte respiratory burst, which points to monocytes as both target and actor in the immune dysregulation associated with chronic uremia.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium iodide, puriss. p.a., reag. ISO, reag. Ph. Eur., ≥99.0%
Sigma-Aldrich
Potassium iodide, ACS reagent, ≥99.0%
Sigma-Aldrich
Potassium iodide, ReagentPlus®, 99%
Sigma-Aldrich
Potassium iodide, puriss., meets analytical specification of Ph. Eur. BP, USP, 99.0-100.5% (calc. to the dried substance)