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Spatial mapping and quantification of soft and hard protein coronas at silver nanocubes.

Nano letters (2014-03-13)
Teodora Miclăuş, Vladimir E Bochenkov, Ryosuke Ogaki, Kenneth A Howard, Duncan S Sutherland
ABSTRACT

Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethylene glycol, anhydrous, 99.8%
Sigma-Aldrich
Silver trifluoroacetate, 98%