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Improvement of preimplantation development of in vitro-fertilized bovine zygotes by glucose supplementation to a chemically defined medium.

The Journal of veterinary medical science (2014-07-01)
Nobutada Sakagami, Osamu Nishino, Satoshi Adachi, Hidenobu Umeki, Hiroko Uchiyama, Kyoko Ichikawa, Kazuhisa Takeshita, Etsushi Kaneko, Kiyoshi Akiyama, Shuji Kobayashi, Hiromichi Tamada
ABSTRACT

The influences of glucose supplementation on early development of bovine embryos in BSA-free synthetic oviduct fluid were examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose either at 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in the group supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controls without glucose supplementation. The results suggest that appropriate amounts of glucose supplemented to the medium at the specific stage of embryo culture may be useful for the production of bovine blastocysts.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Estradiol, analytical standard
Sigma-Aldrich
Poly(vinyl alcohol), 87-90% hydrolyzed, average mol wt 30,000-70,000