Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions.

Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions.

Bioorganic & medicinal chemistry letters (2001-10-26)

F Tanaka, L Kerwin, D Kubitz, R A Lerner, C F Barbas

ABSTRACT

We developed a visible detection system for antibody-catalyzed retro-aldol-retro-Michael reactions. Aldolase antibody 38C2 catalyzed the reaction of substrate 1 to provide 6-bromo-2-napthol that forms a visible colored azo dye with diazonium salts. This system has potential for the screening of novel catalysts.

MATERIALS

Product Number

Brand

Product Description

B73406

Sigma-Aldrich

6-Bromo-2-naphthol, 97%