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Biotinylated proteins as molecular weight standards on Western blots.

Analytical biochemistry (1986-02-01)
D Della-Penna, R E Christoffersen, A B Bennett
ABSTRACT

Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase complex and reaction with 4-chloro-1-naphthol and hydrogen peroxide. The biotinylated proteins are easy to prepare and are useful as molecular weight standards with most procedures employing immunodetection of proteins following transfer to nitrocellulose paper.

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Product Description

Sigma-Aldrich
Biotinylated Molecular Weight Marker, mol wt 6,500-180,000 Da