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Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.

Journal of the National Cancer Institute (1979-04-01)
I C Summerhayes, L M Franks
PMID107359
ABSTRACT

Neoplastic transformation of C57BL/lcrf-a' mouse bladder epithelium was induced in long-term primary cultures by a single 24-hour treatment with 7,12-dimethylbenz[a]anthracene on day 2 of culture. Transformed foci appeared earlier (40--60 days) and at a higher frequency (28%) in cultures from old donors (28--30 mo) compared with 100 days and 0.9% in cultures from young adult donors (5--7 mo). After transplantation into syngeneic mice, transformed cells produced carcinomas. Spontaneous epithelial transformation occurred in dimethyl sulfoxide-treated old cultures after the same interval (40--60 days) as in the carcinogen-treated cultures but at a lower frequency (5.3%). Spontaneous epithelial transformation did not occur in cultures from young donors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MB49 Mouse Bladder Carcinoma Cell Line, MB49 mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.