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Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery.

Journal of materials chemistry. B (2019-08-01)
Sandra Jimenez-Falcao, Natalia Joga, Alba García-Fernández, Antoni Llopis Lorente, Daniel Torres, Beatriz de Luis, Félix Sancenón, Paloma Martínez-Ruiz, Ramón Martínez-Máñez, Reynaldo Villalonga
ABSTRACT

We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

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(3-Iodopropyl)trimethoxysilane, ≥95.0% (GC)