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Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism.

Cell host & microbe (2019-08-06)
Won Jong Kim, Dustin Higashi, Maira Goytia, Maria A Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A McLean, Joseph Duncan, David Trees, Ann E Jerse, Magdalene So

The mucosa is colonized with commensal Neisseria. Some of these niches are sites of infection for the STD pathogen Neisseria gonorrhoeae (Ngo). Given the antagonistic behavior of commensal bacteria toward their pathogenic relatives, we hypothesized that commensal Neisseria may negatively affect Ngo colonization. Here, we report that commensal species of Neisseria kill Ngo through a mechanism based on genetic competence and DNA methylation state. Specifically, commensal-triggered killing occurs when the pathogen takes up commensal DNA containing a methylation pattern that it does not recognize. Indeed, any DNA will kill Ngo if it can enter the cell, is differentially methylated, and has homology to the pathogen genome. Consistent with these findings, commensal Neisseria elongata accelerates Ngo clearance from the mouse in a DNA-uptake-dependent manner. Collectively, we propose that commensal Neisseria antagonizes Ngo infection through a DNA-mediated mechanism and that DNA is a potential microbicide against this highly drug-resistant pathogen.

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Sodium bicarbonate/Sodium carbonate concentrate, Na2CO3 36 mM and NaHCO3 34 mM in water, IC eluent concentrate (20x) for Metrosep A Supp 4
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Chloramphenicol, ≥98% (HPLC)
2-Propanol, BioReagent, for molecular biology, ≥99.5%
Erythromycin, BioReagent, suitable for cell culture
Bovine Serum Albumin, lyophilized powder, ≥96% (agarose gel electrophoresis)
Agarose, Ultra-low Gelling Temperature, molecular biology grade