Concentrate your protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters. Amicon® ultrafiltration devices provide a fast, reliable method to concentrate proteins and other biomolecules, separate components in biological and environmental samples by molecular weight, and desalt and dialyze proteins and antibodies for sample preparation. We’ve engineered Amicon® Ultra filters with low-binding Ultracel® regenerated cellulose membranes to ensure more precise molecular weight cut-offs and high protein recovery.
Amicon® Ultra centrifugal filter devices provide fast ultrafiltration with high concentration factors from dilute and complex sample matrices. The vertical design and membrane surface area provide fast sample processing, high sample recovery, and the capability for up to 80-fold concentration factors.
Amicon® Ultra centrifugal devices are offered with nominal molecular weight limits (NMWLs) of 3 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa in four different volumes:
Amicon® Pro Adapters are designed for use with Amicon® Ultra centrifugal filtration devices to enable multiple simultaneous protein workflow processes including, but not limited to, dialysis, diafiltration, affinity purification, buffer exchange, filter-aided sample preparation (FASP), and concentration. The Amicon® Pro Adapter is the only product on the market that offers the flexibility to perform any or all protein workflow manipulations in a singledevice for maximum recovery without sample transfer.The uniquely designed interface between the exchange tube tip and the Amicon® Ultra device enables greater than 99% buffer exchange in a single spin.
The Amicon® Pro Adapter is comprised of a 10 mL chamber for wash and buffer exchange steps, a frit that allows the insertion of affinity resins and simultaneous elution and concentration of proteins and antibodies, and an exchange tip that regulates flow for controlled, continuous diafiltration.
Amicon® Stirred Cells use pressurized ultrafiltration (pUF) at high flow rates to process large sample volumes for rapid concentration, diafiltration, and buffer exchange. Gas pressure is applied directly to the cell for filtration. Compatible membrane filters includeUltracel® RC (regenerated cellulose) andBiomax® PES (polyethersulfone) membranes, offered in a broad range of nominal molecular weight limits ranging from 1kDato 500kDa. Solutes above the membrane’s nominal molecular weight cut-off (NMWCO) are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.