MilliporeSigma
HomeProductsLabwareCell Culture & CryogenicsMillicell® Cell Culture Inserts & Plates

Millicell® Cell Culture Inserts & Plates

Millicell® cell culture inserts, plates, and cultureware are designed to maximize user convenience and biological relevance in cell culture applications. Millicell® permeable inserts encourage the natural morphology and proliferation of adherent and suspension cell lines. Optimized membranes provide cells with structure and function that closely mimic their in vivo environment, leading to more biologically-relevant results for applications including toxicity screening, primary and secondary screening, cell signaling, cell proliferation, transport assays, and ADME/Tox drug safety studies. Millicell® HY multilayer flasks save time and space in the incubator when propagating cells. Our Millicell® EZ SLIDE culture chamber slides bring enhanced features and unique design that help you spend less time reseeding cells and struggling with difficult-to-use devices, and more time analyzing results.

Millicell® inserts and plates

In vivo, cells live in a three-dimensional environment and can access nutrients from all directions. In contrast, traditional plastic culture plates force cells to grow on a smooth, two-dimensional surface, leading to flattened nuclei and poor function. Millicell® inserts and plates feature a design that allows access to both the basolateral and apical surfaces of cell layers. This promotes natural, multidimensional growth and provides more relevant in vitro models than traditional plastic plates.

Millicell® cultureware for biologically relevant results
  • Millicell® Hanging Inserts can be suspended within plate wells by innovatively-designed flanges that position the insert off-center, facilitating pipetting by creating a larger space to one side of the well. Millicell® hanging inserts make it possible to study both apical and basal surfaces of the cell monolayer, and are excellent tools for coculture, as there is no disturbance of the outer basolateral cell culture by the presence of insert feet.
    • For coculture and permeability assays
    • Unique design facilitates basolateral access and reduces risk of contamination
    • PET (polyethylene terephthalate) track-etched, translucent thin film membrane for enhanced cell visualization and monitoring of the cell monolayer.
    • Available in three well sizes and five membrane pore sizes, including a 1 µm pore size that is optically transparent for better visualization by microscopy
    • Compatible with fluorophores for fluorescent imaging
  • Millicell® Standing Inserts are free-standing inserts that can rest at the bottom of cell culture plate wells and other vessels, elevated by insert feet. These standing inserts promote the natural growth of suspension and adherent cell lines and are available in three different optimized membranes tailored for specific needs.
    • Biopore™ PTFE (polytetrafluoroethylene) membranes are low protein-binding membranes developed for live cell viewing and immunofluorescent applications. The transparent membrane exhibits low background fluorescence compared to other membrane materials and is suitable for attachment-dependent cells when coated with an extracellular matrix.
    • MF-Millipore™ MCE (mixed cellulose esters) membranes are Triton®-free and can be used for cell surface receptor, in vitro toxicology, microbial attachment, and polarized uptake assays.
    • Isopore™ PCF (polycarbonate) membranes are hydrophilic and tissue culture-treated to allow growth of adherent phenotype cells without the use of extracellular coating matrix (ECM). The high pore density allows for maximum diffusion in drug transport and uptake assays.
  • Millicell® Organotypic Standing Inserts are designed for superior study of three-dimensional explant structures.
    • For high cell viability and superior study of three-dimensional explant structures
    • Shorter profile allows inserts to fit inside a standard petri dish
    • Optically clear Biopore™ (PTFE) membrane supports high viability and excellent trans-membrane oxygen transport, and is optimized for long-term explant maintenance
  • Millicell® Plate Assemblies enable cell growth, feeding, and analysis in one membrane-bottom plate optimized to grow and sustain high-integrity cell monolayers. These plates can be used manually or with automated cell seeding, feeding, and washing systems. Their thoughtful design improves function with seed-and-feed systems, automated liquid handling systems, and transepithelial electrical resistance (TEER) measurement instruments.
    • Complete system with a multi-well membrane-bottom plate, single-well or multi-well receiver tray, and lid
    • Apical assist protects the cell monolayer and allows for easier pipetting and basolateral access
    • Available in a variety of polyethylene terephthalate (PET) membrane pore sizes.
    • Teardrop-shaped receiver wells eliminate air bubbles
    • Raised well edges for improved tape sealing and large font labeling for easy well identification
Millicell® EZ slides for all-in-one culturing, fixing, staining, and viewing of cells

Millicell® EZ Slides

Millicell® EZ Slides can be used to culture, fix, stain and view your cells all in one device. No need to tediously move cover slips from your culture dish to a slide, or to remove the media chamber from the slide prior to fixing or staining. Easily remove the chamber structure without worrying about breaking slides or ruining cell layers. Acquire data simply and quickly with Millicell® EZ Slides.

  • Breakable tabs for easy well removal
  • No tools required to remove wells from slide
  • No adhesive used to attach wells to slide
  • No leaky wells
  • Polypropylene wells facilitate fixing and staining with wells still attached, allowing different probes in wells
  • Slide holder protects slide from damage
Millicell HY high yield flask

Millicell® HY Flasks

Innovative multilayer cell culture flasks support uniform, consistent cell growth for high cell recovery. Manufactured with multiple identical layers, Millicell® HY flasks maximize cell culture surface area for the highest yields, while saving incubator space.

  • Save time spent passaging cells
  • Save costs associated with media, cells, and reagents
  • Consistently high cell yields
  • Uniform health across all layers
  • Easy transition from single-layer cell culture flasks
  • Fits under microscopes for monitoring cell health and confluency
  • Stackable design saves incubator space

Products

Sort by Relevance
Showing 1-20 of 43
Page 1 of 3
Page 1 of 3
Showing 1-20 of 43
Product Number
Product Description
Pricing
PICM0RG50
Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
PICM03050
Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
PEZGS0816
Millicell EZ SLIDE 8-well glass, sterile, Simplify your cell analysis by using the Millicell EZ SLIDE 8 well glass slide to culture, fix, stain & view your sample all in one device. There’s no need to remove the media chamber from the microscope slide prior to fixing or staining.
PSHT004
Millicell-96 Cell Culture Insert Plate, Polycarbonate, 0.4 μm pore size, sterile, 96-well cell culture plate, single-well feeder tray, 96-well receiver tray and lid
PIHP01250
Millicell Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm, Polycarbonate cell culture insert with pore size of 0.4 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PICM01250
Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm, Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 24-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
PFHYS1008
Millicell HY 5-layer cell culture flask, T-1000, sterile, Millicell HY 5-layer flask, T-1000, sterile high yield cell culture flasks provide a consistent, quality growth environment across all layers, with the same volume of media in each layer & the same media requirements as traditional T-flasks.
PSHT010R5
Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 µm, Polycarbonate cell culture insert with pore size of 0.4 µm used in a 24-well pate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PSHT004S5
Millicell-96 Cell Culture Insert Plate, Polycarbonate, 0.4 μm pore size, sterile, 96-well cell culture plate, 96-well receiver tray and lid, pack of 5
PEZGS0416
Millicell® EZ Slide, 4-well, Glass Chamber Slide, Sterile
PSMW010R5
24 well Collection Tray, 24-well receiver trays with lids for Millicell®-24 Cell Culture Insert Plates, sterile, pack of 5
PI8P01250
Millicell Cell Culture Insert, 12 mm, polycarbonate, 8.0 µm, Polycarbonate cell culture insert with pore size of 8.0 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PIHP03050
Millicell Cell Culture Insert, 30 mm, polycarbonate, 0.4 µm, Polycarbonate cell culture insert with pore size of 0.4 µm used in a 6-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PFHYS0616
Millicell® HY Flask, 3-Layer, T-600, Sterile, Cell Culture Flask
PSRP010
Millicell-24 Cell Culture Insert Plate, polyethylene terephthalate, 1.0 µm, Polyethylene terephthalate, 1.0 μm pore size, sterile, 24-well cell culture plate, single-well feeder tray, 24-well receiver tray and lid
PITP01250
Millicell Cell Culture Insert, 12 mm, polycarbonate, 3.0 µm, Polycarbonate cell culture insert with pore size of 3.0 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PIXP01250
Millicell Cell Culture Insert, 12 mm, polycarbonate, 12 µm, Polycarbonate cell culture insert with pore size of 12.0 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
PEZGS0896
Millicell EZ SLIDE 8-well glass, sterile, Simplify your cell analysis by using the Millicell EZ SLIDE 8 well glass slide to culture, fix, stain & view your sample all in one device. There’s no need to remove the media chamber from the microscope slide prior to fixing or staining.
PSHT010R
Millicell-24 Cell Culture Insert Plate, Polycarbonate, 0.4 μm pore size, sterile, 24-well cell culture plate, single-well feeder tray, 24-well receiver tray and lid
PSSW010R5
Single-well Receiver Tray with Lid, Single-well feeder trays with lids for Millicell® Cell Culture Insert Plates, sterile, pack of 5
Page 1 of 3
Page 1 of 3

Related Product Resources

Article: Alternative High-Yield Cell Culture Systems
Brochure: Multiply Your Success MultiScreen® and Millicell® Filter Plates for Assay Development

Related Product Categories

Classical Media & Buffers
Cell culture media formulations have been optimized over decades of research in response to diverse cellular physiology. Select media such as DMEM, MEM, RPMI-1640, Ham’s F-10 & F-12 formulations, as well as serum-free solutions such as M199.
Multiscreen® Filter Plates
MultiScreen® filtration plates in 96-well and 384-well formats with a variety of membrane options are for high throughput screening, ELISpot assays, ligand binding assays, cell-based assays, ADME testing, PCR cleanup, and sample prep.
Millipore® Sterile Filters
Prepare cell culture media and filter-sterilize aqueous solutions from a few mL to 20 L using industry-leading Millipore® sterile filtration devices. Choose fit-for-application membranes and pore sizes in Steriflip®, Sterivex®, Stericap®, and Steripak® devices.
Stericup® Vacuum Filters
Essential Stericup® and sustainable Stericup® E filters are integral devices for filtering media to protect cell cultures. Choose from membranes and pore sizes for fast flow, low protein binding, mycoplasma exclusion, and plastic waste reduction.
Cell Counting Equipment
Accurate, consistent cell counting is critical for cell culture and downstream cellular assays and experiments. A handheld coulter counter provides the most accurate and reproducible cell counts in seconds. Disposable hemocytometers reduce risk in infectious settings.
CellASIC® ONIX2 Microfluidics
The CellASIC® ONIX2 Microfluidic System is a powerful, automated platform that allows precise manipulation of the cell culture environment for live-cell imaging and analysis using mammalian, yeast, and bacterial cells.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.

Related Applications

Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Immunohistochemistry
Immunohistochemistry (IHC) is a common immunostaining technique that is used by researchers to identify specific antigens of interest in healthy and diseased tissues.
Electron Microscopy
Electron microscopy utilizes a scanning or transmission system to pass an electron beam through a specimen to obtain ultrahigh resolution images with detailed structural information at the surface or atomic level.

Highlights

Slide 1 of 0