Thrombin from bovine plasma

powder, suitable for cell culture, ≥1,500 NIH units/mg protein (E1%/280 = 19.5)

Factor IIa
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:



specific activity

≥1,500 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~31 kDa
light chain ~6 kDa


cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

cow ... F2(280685)

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General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.


Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units, and is measured by direct comparison to an NIH thrombin reference standard.


When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.

Analysis Note

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Certificate of Analysis

Certificate of Origin

Zachary T Britton et al.
Protein expression and purification, 84(2), 224-235 (2012-06-23)
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Clinical chemistry and laboratory medicine, 50(9), 1601-1605 (2012-10-27)
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