All Photos(4)

PLN70

Sigma-Aldrich

GenElute Plasmid Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonym(s):
Gen Elute, GenElute Plasmid Kit
NACRES:
NA.52

Quality Level

usage

sufficient for 70 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

storage temp.

15-25°C

Looking for similar products? Visit Product Comparison Guide

General description

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK Germany

WGK 2

Certificate of Analysis

Certificate of Origin

Cloning and Characterization of Serine-rich Entamoeba histolytica Protein Gene from an Iranian E. histolytica Isolate
Sima Rasti
Pakistan journal of biological sciences: PJBS, 9, 654-658 (2006)
Comparison of three molecular methods for the detection of pilchard herpesvirus in archived paraffin-embedded tissue and frozen tissue.
Crockford M
Diseases of Aquatic Organisms, 82(1), 37-44 (2008)
Genetic determinants of resistance to fusidic acid among clinical bacteremia isolates of Staphylococcus aureus.
Lannergard J
Antimicrobial Agents and Chemotherapy, 53(5), 2059-2065 (2009)
Masa-aki Yoshida et al.
BMC evolutionary biology, 11, 180-180 (2011-06-28)
Coleoid cephalopods (squids and octopuses) have evolved a camera eye, the structure of which is very similar to that found in vertebrates and which is considered a classic example of convergent evolution. Other molluscs, however, possess mirror, pin-hole, or compound...
Veerle Derous et al.
BMC research notes, 4, 213-213 (2011-06-24)
A number of allele replacement methods can be used to mutate bacterial genes. For instance, the Red recombinase system of phage Lambda has been used very efficiently to inactivate chromosomal genes in E. coli K-12, through recombination between regions of...

Protocols

Escort™ IV Transfection Reagent Protocol

Escort IV Transfection Reagent Protocol

Escort™ III Transfection Reagent Protocol

Escort™ III Transfection Reagent Protocol

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service