All Photos(4)

NA1020

Sigma-Aldrich

GenElute PCR Clean-Up Kit

sufficient for 70 purifications

Synonym(s):
PCR Purification, Gen Elute
NACRES:
NA.52

Quality Level

usage

sufficient for 70 purifications

storage temp.

15-25°C

General description

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Application

Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
GenElute PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.

Features and Benefits

  • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
  • Recovers up to 95% of PCR products between 100 bp and 10 kb
  • Removes over 99% of primers and other components
  • Eliminates the need to remove mineral oil by organic extraction
  • 40% more purification preps supplied than market leader

Other Notes

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Principle

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product NA1020, GenElute PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?

    Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use

  6. Can Product No. NA1020, GenElute PCR Clean-Up Kit,  be used to clean up PCR products larger than 10 kb?

    We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well.  That said, the bound DNA might be more difficult to elute.  Heating the elution solution to 60-65°C may help with elution.  Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.

  7. When using Product No. NA1020, GenElute PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?

    The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.

  8. When using GenElute PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?

    One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.

  9. Can GenElute PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?

    This kit will work with labeled PCR products and no modifications to the standard protocol are needed.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Andreas Veith et al.
Frontiers in microbiology, 2, 37-37 (2011-07-13)
The sulfur oxygenase reductase (SOR) is the initial enzyme of the sulfur oxidation pathway in the thermoacidophilic Archaeon Acidianus ambivalens. The SOR catalyzes an oxygen-dependent sulfur disproportionation to H(2)S, sulfite and thiosulfate. The spherical, hollow, cytoplasmic enzyme is composed of...
Kathleen Gärtner et al.
Retrovirology, 6, 32-32 (2009-04-08)
Foamy viruses (FVs) are the most genetically stable viruses of the retrovirus family. This is in contrast to the in vitro error rate found for recombinant FV reverse transcriptase (RT). To investigate the accuracy of FV genome copying in vivo...
Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization.
Vandewoestyne M
International Journal of Cancer. Journal International Du Cancer, 130(5), 1098-1108 (2012)
T Nobre et al.
Proceedings. Biological sciences, 277(1680), 359-365 (2009-10-16)
The mutualism between fungus-growing termites (Macrotermitinae) and their mutualistic fungi (Termitomyces) began in Africa. The fungus-growing termites have secondarily colonized Madagascar and only a subset of the genera found in Africa is found on this isolated island. Successful long-distance colonization...
Maha Alriyami et al.
Genome research, 29(12), 1951-1961 (2019-11-07)
Concordance for type 1 diabetes (T1D) is far from 100% in monozygotic twins and in inbred nonobese diabetic (NOD) mice, despite genetic identity and shared environment during incidence peak years. This points to stochastic determinants, such as postzygotic mutations (PZMs)...

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