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05-100-I

Sigma-Aldrich

Anti-HDAC1 Antibody, clone 2E10

clone 2E10, Upstate®, from mouse

Synonym(s):

HDAC1, Histone deacetylase 1, HD1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2E10, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_032254

UniProt accession no.

O09106

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HDAC1(3065)
mouse ... Hdac1(433759)

General description

Histone deacetylase 1 is a nuclear enzyme encoded by the Hdac1 gene (also known as HD1, RPD3, MommeD5, Hdac1-ps) in mouse and HDAC1 (also known as GoON-10, RPD3L1) gene in human. Hdac1 plays an important role in epigenetic regulation of gene expression by catalyzing the hydrolysis of N(6)-acetylated histone lysine residues. Hdac1 is also known to deacetylate p53 and thereby downregulate p53 activity.

Immunogen

KLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.

Application

Anti-HDAC1 Antibody, clone 2E10 is an antibody against HDAC1 for use in Western Blotting, Immunoprecipitation.
Immunoprecipitation Analysis A representative lot was used to immunoprecipitate HDAC1 from HeLa cell lysate, followed by immune complex enzyme activity assay using HDAC Assay Kit (Fluorometric Detection), Cat. No. 17-356.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HDAC1 in 10 µg of A431 cell lysate.

Target description

~55 kDa observed

Linkage

Replaces: 05-100

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria Rivas et al.
Cellular and molecular gastroenterology and hepatology, 12(5), 1669-1682 (2021-07-11)
Epigenetic regulation of gene expression plays a critical role in the development of liver cancer; however, the molecular mechanisms of epigenetic-driven liver cancers are not well understood. The aims of this study were to examine molecular mechanisms that cause the
Tarin M Bigley et al.
Journal of virology, 87(13), 7393-7408 (2013-04-26)
Human cytomegalovirus (HCMV) is a common agent of congenital infection and causes severe disease in immunocompromised patients. Current approved therapies focus on inhibiting viral DNA replication. The HCMV kinase pUL97 contributes to multiple stages of viral infection including DNA replication

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