MilliporeSigma
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04-817

Sigma-Aldrich

Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

culture supernatant, clone MC463, Upstate®

Synonym(s):
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

MC463, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine

Specificity

Broad species cross-reactivity is expected
Histone H3 phosphorylated at Serine 10

Immunogen

Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3

Application

Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.
Western Blot Analysis:
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).

Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.

Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.


Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Quality

routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells

Target description

17kDa

Linkage

Replaces: 05-817

Physical form

Cultured supernantant in 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Quality

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