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L2254

Lipoprotein Lipase from bovine milk

ammonium sulfate suspension, ≥2,000 units/mg protein (BCA)

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Synonym(s):

Diacylglycerol lipase, Diacylglycerol acylhydrolase

CAS Number:

9004-02-8

Enzyme Commission number:

3.1.1.34 (BRENDA, IUBMB)

EC Number:

232-669-1

MDL number:

MFCD00131523

NACRES:

NA.54

Quality Level

200

biological source

bovine milk

form

ammonium sulfate suspension

specific activity

≥2,000 units/mg protein (BCA)

storage temp.

2-8°C

General description

Lipoprotein Lipase (LPL) from bovine milk is a glycoprotein. It exists as a homodimer and comprises two N-linked oligosaccharides. It is heat-labile.

Application

Lipoprotein Lipase from bovine milk has been used:

as a supplement to test its effect on DiI (1,1′-dioctadecyl-3,3,3′-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells
to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)
to test its effect on gene expression in normal human astrocytes

Packaging

5000 units in glass bottle

Biochem/physiol Actions

Lipoprotein Lipase (LPL) from bovine milk contributes to maximal lipolytic activity. It associates with casein micelle. LPL regulates triglyceride utilization and displays positional specificity. Lipases, in general, catalyzes the lipolysis of triglycerides especially at the fatty acid in sn-1 and sn-3 positions of the triglyceride.

Unit Definition

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.

Physical form

Suspension in 3.8 M ammonium sulfate, 0.02 M Tris HCl, pH 8.0

Preparation Note

Affinity purified

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Technical Information

(L2254) - Enzyme Assay