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UC0E01

Sigma-Aldrich

UCOE® Single Expression Puromycin Vector Set

Synonym(s):
UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element , UCOE01
eCl@ss:
34360190

Quality Level

shipped in

dry ice

General description

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HS-puro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AS-puro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HS-puro-SceI and CET 1019 AS-puro-SceI vectors possess the following features:
  • A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzymes to permit easy insertion of any gene of interest (cDNA or genomic)
  • A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
  • A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
  • A β-lactamase gene for plasmid selection and propagation in the transformed prokaryotic cells in the presence of ampicillin
  • An I-Scel homing restriction endonuclease site for linearization of constructs prior to the transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes


THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

Application

Research Category
General Cell Biology

All
Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.
Research Sub Category
All

Components

1) 10ug CET 1019 HS-puro-SceI (+) Vector (CS221296)

2) 10ug CET 1019 AS-puro-SceI (+) Vector (CS221284)

3) 10ug SC HS-puro (-) Vector (CS221286)

4) 10ug SC AS-puro (-) Vector (CS221287)

Storage and Stability

Plasmids should be stored at -20C. Avoid repeated freeze-thaw events.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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