Characterization of Monoclonal Antibodies by SPR

WEBINAR

Detailed description:

An important characteristic of an antibody is its Fc effector function. Antibodies can be engineered to obtain the desired binding of the Fc region to Fc receptors expressed on effector cells. Hence, it is crucial to evaluate the binding interaction of mAbs/ADC with Fc receptors in the early phase of drug development to understand the potential biological activity of the product in vivo.

Surface Plasmon Resonance (SPR) is a powerful technique to establish binding kinetics in real-time, label-free, and high sensitivity with low sample consumption. Along with target antigen binding, it is crucial to evaluate the binding interaction of antibodies and ADCs with Fc receptors. Our SPR case studies investigated the impact on binding kinetics of ADCs with different linkers and the binding interactions of SARS-CoV-2 spike protein variants and evaluated the neutralizing ability of therapeutic mAbs. SPR characterization can be facilitated in all stages of the product life cycle to ensure the quality and safety of mAbs and ADCs.

Highlights of this webinar:

• Surface plasmon resonance as a powerful tool for biologic characterization including mAbs and ADCs.
• SPR allows rapid binding analysis in real time without using labels for SARS-CoV-2 receptor binding domain mutations.
• Kinetic data is indicative of possible neutralizing activity allowed assessment of neutralizing ability of therapeutic monoclonal antibodies.
• The application can provide preliminarily efficacy information and facilitated mAbs/ACDs candidate selection process.