主页产品细胞培养和分析细胞分析活细胞成像试剂

活细胞成像试剂

实时的活细胞成像可使科学家能够对无法使用传统的终点技术（例如固定成像和PCR仅能捕获一个静态的时刻）观察的动态细胞事件进行监测。我们产品的活细胞成像试剂组合包括用于细胞示踪的新型荧光细胞标记技术、荧光慢病毒生物传感器、传统荧光染料和用于实时分析细胞事件的探针。这些试剂适于通过CellASIC^® ONIX2微流体活细胞成像系统等实时孵育和成像系统捕获动态细胞事件。

PKH和CellVue^®荧光细胞连接子标记物

PKH和CellVue^®细胞荧光连接试剂盒可为长时间的活细胞成像实验提供活细胞荧光膜标记。

PKH和CellVue^®染料主要特点：

对体内活细胞稳定长达100天
对于真核细胞、细菌细胞和寄生虫是安全的
无明显的染料漏出或细胞间转移
信号均匀且可重现
利用光谱不同的染料可实现多色分析
兼容其他荧光标记物
已被7,000多份出版物所引用

采用BioTracker NIR650溶酶体染料和BioTracker 488 Green线粒体染料共染的活HeLa细胞。

LentiBrite™荧光慢病毒生物传感器

LentiBrite™荧光慢病毒生物传感器是一套预包装的慢病毒颗粒，编码自噬、凋亡和细胞结构相关荧光蛋白，使活细胞中的这些过程分析可视化。

慢病毒生物传感器的特点：

预包装、即用型形式、标记了单体GFP和RFP
每管最低滴度（≥3 x 108 IFU/mL）
转染效率优于化学方法
适用于分裂中、未分裂以及难转染的细胞，如原代细胞或干细胞
不破坏细胞功能

BioTracker活细胞荧光染料和染色剂

BioTracker活细胞染料是一类细胞可渗透性染料，适用于细胞器标记、细胞凋亡检测、细胞活力和细胞健康分析、缺氧监测、活性氧种类示踪、钙指示剂功能以及神经细胞和干细胞培养等活细胞应用。

BrightCell™光稳定培养基

BrightCell™光稳定培养基产品包括细胞培养基和补充剂，可在细胞长时间暴露于荧光显微镜所用的高能光照期间为其提供出色的健康支持。BrightCell™培养基产品还表现出了极低水平的自发荧光和光漂白，能够显著提高荧光活细胞成像的数据质量。

MEMO^®培养基专用于在细胞暴露于蓝光之前和期间替代DMEM
NEUMO^®培养基专为神经元细胞培养而开发，可在活细胞成像过程中替代Neurobasal培养基
SOS™补充剂可替代B-27^®、NS21、SATO和N2等光毒性无血清补充剂，从而在活细胞成像过程中维持必需的蛋白质

Luminicell Tracker™荧光纳米粒子

LuminiCell Tracker™荧光纳米粒子为长期的细胞追踪实验提供了一种有效、简单、无毒的试剂。这种细胞追踪染料具有聚集诱导发光(AIE)特性，封装在生物相容性、细胞可渗透的荧光纳米粒子内（AIE纳米点），可提供增强的光稳定性、超亮荧光和低细胞毒性，适用于较长时间的生物成像应用。

荧光更强：是很多细胞标记技术的10倍
光稳定性：荧光信号不淬灭的时间延长3倍
生物相容性：无毒性有机纳米粒子适合生物学应用
快速标记：简单易行的4小时内细胞标记

Long-Term Live Cell Tracking of Cancer and Stem Cells Using a Biocompatible Fluorescent Nanoparticle Based Upon Aggregation Induced Emission (AIE Dot) Nanotechnology

Biocompatible organic fluorescent nanoparticles (AIE Dots) that can be used to label cells and vasculature for long-term live cell tracking and tracing experiments.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for microvesicle tracking experiments.

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Cancer Stem Cell Detection Utilizing a Red-shifted Fluorescent Live Cell Probe for Aldehyde Dehydrogenase (ALDH), AldeRed™ 588-A.

AldeRed™ 588-A is a red fluorescent live cell probe that detects ALDH activity used to identify cancer stem cells and progenitor cells in culture. Cancer stem cells (CSCs) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis. Some researchers believe that cancer arises from cancer stem cells that originate as a result of mutational hits on normal stem cells.

Highlights

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Live Cell Imaging of Hypoxia in Cancer Cells using the CellASIC® ONIX2 Microfluidic System

A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC® ONIX2 System. After overnight incubation, cells were perfused with complete DMEM media without dye at 1 psi under hypoxic conditions (0% O2) for 16 hours at 37°C. Fluorescent images were captured over 16 hours using 465 nm excitation, and a 525 nm emission filter. The BioTracker 520 Green Hypoxia Dye increases in fluorescence intensity with decreasing oxygen levels (increased hypoxic conditions).

Live Cell Imaging of β-Actin Cytoskeleton Proteins using LentiBrite™ Fluorescent Biosensors

U2OS cells were plated in a chamber slide and transduced with LentiBrite™ GFP-β-actin lentiviral particles at an MOI of 20 for 24 hours. After media replacement and an additional 48 hours of further incubation, the cells were treated with 2 µM cytochalasin-D for another 2 hours, to induce actin depolymerization. The GFP- β -actin displays a filamentous distribution in the untreated cells, and upon actin depolymerization, adopts a punctate distribution. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Autophagy using LentiBrite™ Fluorescent Biosensors

HT-1080 cells were plated in a chamber slide and transduced with LentiBrite™ GFP-LC3 lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.

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