微生物细胞培养
微生物细胞培养不仅在分子生物学中用于克隆及重组蛋白表达，而且还可在临床应用中分离、检测和鉴定致病微生物。其原理是通过控制实验室条件来促进细胞生长和分裂。细菌及其他微生物可使用无菌技术来防止污染在液体肉汤或固体营养琼脂培养基中进行培养。
克隆与重组蛋白表达
细菌培养可用于重组蛋白表达、质粒克隆和扩增以及细菌人工染色体（BAC）和粘粒的克隆。细菌细胞在经转化改造后可摄入和吸收外源基因和质粒。化学处理后的感受态细胞一般通过电穿孔或热激来实现转化。
转化完成后细菌既可根据重组质粒DNA所赋予的获得性抗生素抗性进行鉴定和选择，也可以使用“蓝白斑”筛选对表达β-半乳糖苷酶作为标记的重组细菌进行鉴定。酵母也可以通过电穿孔和其他方法进行转化，用于形成酵母双杂交和检测报告基因，进而研究蛋白质的表达和相互作用。
用于微生物分离和鉴定的培养
微生物培养对于分离、检测和区分引起传染病的细菌和其他微生物具有重要的医学意义。基于培养方法的微生物表征和鉴定同时取决于其表型和基因型。细胞可通过条纹板上的接种来分离细胞产生纯菌落。选择性培养基通过加入抑制剂来抑制某些杂菌的生长以有利于所选菌类的生长。而差异培养基则通过化合物允许科学家仅通过观察菌落外观或代谢或溶血活性差异所引起的周围培养基变化就可以对菌类进行区分。这些培养基主要通过差异化和排除对细菌等微生物进行鉴定。
相关技术文章
- An introduction to microbial media types, uses and applications. Includes formulations and product selection guide.
- Antifoams are supplied as two basic types of composition or mixtures of the two. Organic antifoams are of synthetic origin. Silicone-based antifoams are generally considered to be siloxane polymers and are also synthetic.
- Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
- Cell lines for vaccine production and cultivation of SARS CoV-2 and other viruses. Primary, human diploid, and continuous culture cell lines for the propagation of viruses and virus-based vaccines.
- Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.
- 查看完整内容 (21)
相关实验方案
- Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
- Initiating a Starter Culture
- General protocols for growth of competent cells in microbial medium.
- The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
- In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.
- 查看完整内容 (7)
登入以繼續
若要繼續閱讀，請登入或建立帳戶。
還沒有帳戶？