796-0657

HPLC Analysis of Proteins on Discovery^® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

屬性

• 描述: Separated on Discovery^® BIO PolyMA-WAX
• 技術: HPLC: suitable
• 測試參數: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• 適合性: application for HPLC
• 應用: life science and biopharma

描述

分析報告

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

Trypsin Inhibitor