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Key Documents

T9767

Sigma-Aldrich

Trypsin inhibitor

powder, suitable for electrophoresis

同義詞:

SBTI

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About This Item

CAS號碼:
9035-81-8
EC號碼:
232-906-9
MDL號碼:
MFCD00082103
分類程式碼代碼:
12352200
NACRES:
NA.77

product name

胰蛋白酶抑制剂 来源于大豆, For use as a marker in SDS-PAGE, BioReagent

生物源

Glycine max (soybean)

品質等級

200

產品線

BioReagent

形狀

powder

分子量

20,000 Da

包裝

vial of 5 mg

技術

electrophoresis: suitable

溶解度

balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble

運輸包裝

ambient

儲存溫度

2-8°C

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應用

来源于大豆（黄豆）的胰蛋白酶抑制剂用于电泳中标准分子量标记。

生化／生理作用

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

成分

大豆胰蛋白酶抑制剂是一种单体蛋白，在两个二硫键交联的单个多肽链中含有181个氨基酸残基。

單位定義

一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A253所需的酶量。
一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A253所需的酶量。

準備報告

胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。

在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

應用

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描述
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描述
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象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317 - H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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存取文件庫

Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata: diversity, origin and characterization
Godoy MS, et al.
Testing, 8(6), e66689-e66689 (2013)
Selective enrichment of albumin in biological samples by CE using segmental filling with sodium octyl sulfate in the background electrolyte
Lin Chin-Yu and Tseng Wei-Lung
Electrophoresis, 30(3), 532-539 (2009)
Quantification of tear proteins by SDS-PAGE with an internal standard protein: a new method with special reference to small volume tears
Li K, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 248(6), 853-862 (2010)
Isabelle Le Potier et al.
Methods in molecular biology (Clifton, N.J.), 1466, 1-10 (2016-07-31)
Capillary electrophoresis (CE) coupled to fluorescence detection is an invaluable technique for the quantitative analysis of proteins of interest in the field of clinical diagnosis and quality control of novel biotechnology products. The various chemical and instrumental approaches that have
Martín S Godoy et al.
PloS one, 8(6), e66689-e66689 (2013-07-03)
Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands
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文章

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

條款

Trypsin Inhibitors

Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors. Serpins control the activation and catabolism of proteins by the inhibition of serine proteases in vivo.

Trypsin Inhibitors

Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors. Serpins control the activation and catabolism of proteins by the inhibition of serine proteases in vivo.

Chromatograms

application for HPLC

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