ZRB1648

Anti-ATG9A Antibody, clone 5B11, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: APG9-like 1, Autophagy-related protein 9A
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 5B11, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 94.45 kDa; observed mol wt ~110 kDa
• 物種活性: human
• 物種活性（以同源性預測）: canine, bovine
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable using 1:100; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:100; western blot: suitable using 1:1,000
• 同型: IgG
• UniProt登錄號: Q7Z3C6
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ATG9A;APG9L1(79065)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 5B11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Autophagy-related protein 9A. It targets an epitope with in 16 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Autophagy-related protein 9A.

應用

Anti-ATG9A, clone 5B11 ZooMAb, Cat. No. ZRB1648, is a recombinant Rabbit Monoclonal Antibody that specifically targets Autophagy-related protein 9A and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ATG9A in HepG2 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected ATG9A in human spleen and human kidney tissue sections.

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected ATG9A in HepG2 cells.

標靶描述

Autophagy-related protein 9A (UniProt: Q7Z3C6; also known as APG9-like 1, ATG9) is encoded by the ATG9A (also known as APG9L1) gene (Gene ID: 79065) in human. ATG9A is a multi-pass membrane protein that is involved in autophagy and cytoplasm to vacuole transport vesicle formation. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site. Among core autophagy-related proteins, ATG9A is the only multi-spanning transmembrane protein. Both its N- and C-terminal domains are exposed to the cytoplasm. Its N-terminal region (aa 1-36) is important in its role in autophagosome formation and this region is required for both autophagy and its Golgi localization. This region also contains sorting motifs known as the di-acidic motif (aa 5-7), a tyrosine-based motif (aa 8-11), and a di-leucine-based motif (aa 22-27). In mammalian cells, ATG9A is mainly localized to the trans-Golgi network and the endosomal system. It is shown to cycle between them though vesicle trafficking. ATG9A travels through the trans-Golgi network and the endosomal system under nutrient-rich conditions, however, upon induction of autophagy it transiently localizes to the autophagosome. Under amino acid starvation or upon rapamycin treatment, it is shown to redistribute from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. Mutant forms of ATG9A that lack both the tyrosine-based and di-leucine-based sorting motifs exhibit reduced Golgi localization and abnormal accumulation on the recycling endosomes under conditions of nutrient deprivation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Imai, K., et al. (2016). J. Cell Sci. 129(20); 3781-3791).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable