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SCM162

Sigma-Aldrich

DMEM/F-12 PLUS Basal Medium

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

同義詞:

Advanced DMEM/F12, Organoid Basal Media, Organoid Basal Media, DMEMF12, DMEM F12, Dulbecco′s Modified Eagle Medium/Ham′s F-12, Serum-Free Basal Media

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160801

形狀

liquid

技術

cell culture | stem cell: suitable

一般說明

DMEM/F-12 PLUS is an advanced formulation of Dulbecco′s Modified Eagle Medium/Ham′s F-12 basal media that can be used in a broad array of cell lines for applications that require reduced or no serum supplementation. Cell lines cultured in DMEM/F12 PLUS and reduced serum exhibit equivalent or superior cell growth while maintaining the same cell morphology and function as in high serum conditions. Applications for DMEM/F12 PLUS include but are not limited to the following:

• Culture of primary, stem cells and cell lines in applications that require reduced or serum-free medium.
• Optimization of transfections that require reduced or serum-free conditions.
• Production of conditioned medium in reduced serum or serum-free conditions.
• Optimized media for the culture and expansion of multiple 3D organoids and tissues systems.

DMEM/F12 PLUS is a proprietary formulation that contains human serum albumin (HSA), defined lipids, trace elements, chemicals and proteins essential for optimal cell growth in reduced serum conditions. The media contains high-glucose and does not contain L-glutamine or penicillin-streptomycin.

應用

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.
Research Category
Stem Cell Research

品質

Appearance (color): Clear, Red Liquid
Osmolality: 290-330 mOsm
pH: 7.2-7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative

儲存和穩定性

Store at 2-8°C. Before use, aliquot into smaller volumes to avoid contamination. Protect from light when not in use. Acclimate the medium at room temperature for 15-20 minutes before use. Do not warm media at 37°C before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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