ZMS1013

Anti-MAP2 Antibody, clone AP20 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Microtubule-associated protein 2A
• 分類程式碼代碼: 12352203
• NACRES: NA.74

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: AP20, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 217 kDa; observed mol wt ~260 kDa
• 物種活性: rat, mouse, human
• 物種活性（以同源性預測）: Xenopus, bovine, quail, chicken
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: F1MEW3
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: chicken ... Map2(424001) ; frog ... Map2(780043) ; human ... MAP2(4133) ; mouse ... Map2(17756) , Map2(281294) ; rat ... Map2(25595)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone AP20 is a is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects MAP2.

免疫原

Full-length purified microtubule protein (MAP2) from bovine brain.

應用

Anti-MAP2, clone AP20 ZooMAb, Cat. No. ZMS1013, is a recombinant Mouse monoclonal antibody that specifically targets MAP2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAP2 in mouse and rat brain tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in SH-SY5Y cell line and E18 cortical neurons.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected MAP2 in mouse brain and rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Microtubule-associated protein (UniProt: F1MEW3; also known as MAP2) is encoded by the MAP2 gene (Gene ID: 281294) in bovine. MAP2 is a neuron-specific protein that stabilizes microtubules in the dendrites of postmitotic neurons. Its major function is to stabilize neuronal shape by promoting microtubule synthesis and cross-linking with other components of the cytoskeleton. Phosphorylation of MAP2 controls its association with the cytoskeleton and it is developmentally regulated. MAP2 can undergo phosphorylation by GSK-3 and p44/42 MAP kinase. Phosphorylation of MAP2 at threonine 1620 and 1623 by GSK-3 are reported to inhibits MAP2 association with microtubules and affect microtubule stability. MAP2 consists of at least three isoforms produced from a single gene: high molecular mass MAP2a and MAP2b, and low molecular mass MAP2c that is also known as the juvenile MAP2c, which is expressed only during early development and exists mainly in axons. MAP2a appears to replace MAP2c during growth and MAP2b is expressed throughout life. MAP2 can also serve as a marker of synaptic plasticity and its levels are observed to increase after traumatic brain injury. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Soltani, MH., et al. (2005). Am. J. Pathol. 166(6); 1841-1850).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1