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Sigma-Aldrich

REDExtract-N-Amp™血液PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

品質等級

200

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

red

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

REDExtract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR（聚合酶链反应）扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。REDExtract-N-Amp血液PCR试剂盒中含有一种惰性染料，可作为示踪染料以实现方便地将PCR反应加载到琼脂糖凝胶上进行分析。
REDExtract-N-Amp血液PCR试剂盒为从全血中快速提取基因组DNA并通过PCR反应扩增感兴趣的目标基因提供了所有必需的试剂。这种新型的提取系统无需进行任何形式的纯化、有机提取、离心分离、加热、过滤或乙醇沉淀。该试剂盒同时包含一种使用基于抗体的热启动机制，专为从提取物直接扩增而配制的PCR ReadyMix™。REDExtract-N-Amp血液PCR试剂盒含有一种示踪染料，PCR反应物可以方便地上样到琼脂糖凝胶上进行分析。

只需加入Extraction Solution并在室温下孵育5分钟，即可从10 μL全血中提取出基因组DNA。向提取物中加入Neutralization Solution，以便在PCR反应前中和抑制物。随后选取一部分DNA提取物，加入专门配制的PCR ReadyMix。

應用

REDExtract-N-Amp™血液PCR试剂盒已用于从血液样本、外周血白细胞、小鼠宫颈内拭子洗脱液和寄生虫中提取基因组DNA。它还用于通过寡核苷酸引物进行聚合酶链反应（PCR）。

特點和優勢

  • 高效8分钟预制品允许更大的速度和吞吐量
  • 不需要有机提取、离心或酒精沉淀
  • Hot start抗体，用于基因组DNA的高特异性PCR扩增
  • 可与全血或血卡一起使用
  • 在4℃稳定提取至少6个月
  • 兼容任何格式（单管、96孔等）
  • 简单的3步程序，无需特殊设备
  • REDExtract-N-Amp™不需要凝胶分析所需的装载缓冲液或跟踪染料
  • PCR产品适用于直接克隆和测序

原則

只需加入Extraction Solution并在室温下孵育5分钟，即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution，以便在PCR反应前中和抑制物。随后选取一部分DNA提取物，加入专门配制的PCR ReadyMix。

其他說明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律資訊

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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產品號碼
描述
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  • N9784Neutralization Solution for BloodSDS
  • P8240REDExtract-N-Amp™ PCR ReadyMix™ for BloodSDS

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P8115

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血液中和液, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H290,H314

危險分類

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCH5865
SLCF0179
SLCC3911
SLBZ9780
SLBT3583

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存取文件庫

Kosei Moriyama et al.
Microbiology and immunology, 52(7), 375-382 (2008-08-01)
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40
Dongdong Ma et al.
Blood, 118(2), 289-297 (2011-05-19)
HSCs are defined by their ability to self-renew and maintain hematopoiesis throughout the lifespan of an organism. The optical clarity of their embryos and the ease of genetic manipulation make the zebrafish (Danio rerio) an excellent model for studying hematopoiesis.
Slawomir J Teper et al.
Molecular vision, 16, 2598-2604 (2010-12-15)
To determine whether gene polymorphisms of the major genetic risk factor for age-related macular susceptibility 2 (ARMS2 A69S) and the complement factor H Y402H influence the response to a variable-dosing treatment regimen with ranibizumab for age-related macular degeneration. This prospective
Olivier A E Sparagano et al.
Annals of the New York Academy of Sciences, 1081, 505-508 (2006-12-01)
Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy
Anna K Nowińska et al.
Molecular vision, 17, 2333-2342 (2011-09-17)
To analyze genotype-phenotype correlation in patients originating from Polish population with the transforming growth factor beta induced (TGFBI) corneal dystrophies. Sixty affected and 31 unaffected individuals from 15 unrelated Polish families were included in the study. The clinical diagnosis was
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文章

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA blood cards (Whatman) which removes the need for the laborious and costly washing procedures that the standard FTA protocol stipulates. This technique removes the variability between sample preparations and maximizes the number of PCR reactions that can be performed on individual FTA blood card samples.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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