SRP3136
PAF-AH human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
同義詞:
1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PAF acetylhydrolase
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About This Item
分類程式碼代碼:
12352202
NACRES:
NA.32
推薦產品
生物源
human
重組細胞
expressed in HEK 293 cells
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
≤0.1 ng/mL ED50
分子量
47-55 kDa
包裝
pkg of 20 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... PLA2G7(7941)
一般說明
Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55kDa by SDS-PAGE analysis.
生化／生理作用
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet activating factor acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL (low density lipoprotein) particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. High levels of PAF-AH in plasma can be an indication of resistant hypertension. Polymorphism in PAF-AH gene at A379V might be linked with ischemic stroke susceptibility.
序列
FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN
外觀
Lyophilized from 10 mM Tris, pH 8.5.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Hiroyuki Arai
Prostaglandins & other lipid mediators, 68-69, 83-94 (2002-11-16)
Platelet-activating factor (PAF) is one of the most potent lipid mediators and is involved in a variety of physiological events. The acetyl group at the sn-2 position of its glycerol backbone is required for its biological activity, and deacetylation of
Biology of platelet-activating factor acetylhydrolase (PAF-AH, lipoprotein associated phospholipase A2).
Stafforini DM
Cardiovascular Drugs and Therapy, 23, 73-83 (2009)
Platelet-activating factor induces NF-kappa B activation through a G protein-coupled pathway.
Kravchenko VV, et al.
The Journal of Biological Chemistry, 270, 14928-14934 (1995)
Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung.
Honda Z, et al.
Nature, 349, 342-346 (1991)
Association of PLA2G7 gene polymorphisms with ischemic stroke in northern Chinese Han population.
Liu X, et al.
Clinical Biochemistry, 47, 404-408 (2014)
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