SRP2018

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 同義詞: E1A
• 分類程式碼代碼: 12352202
• NACRES: NA.26

屬性

• 生物源: Saccharomyces cerevisiae; canine
• 重組細胞: expressed in E. coli
• 化驗: ≥70% (SDS-PAGE)
• 形狀: frozen liquid
• 分子量: ~33 kDa
• 包裝: pkg of 4 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 100 μg/mL
• 技術: electrophoretic mobility shift assay: suitable
• 顏色: colorless to clear
• NCBI登錄號: J04368; NM_001184062
• UniProt登錄號: P04386
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: Saccharomyces cerevisiae ... GAL4(855828)

描述

生化／生理作用

The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.

外觀

Clear and colorless frozen liquid solution

準備報告

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable