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Key Documents

SRP0676

SAFC

转肽酶 A,金黄色葡萄球菌,His-tag

Staphylococcus aureus, recombinant, N-terminal His-Tag

同義詞:

SrtA, 转肽酶 A

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About This Item

分類程式碼代碼:
41202061

重組細胞

expressed in E. coli

籤條

His tagged

形狀

liquid

分子量

27.7 kDa

UniProt登錄號

儲存溫度

−70°C

一般說明

我们全面的上游工艺化学品系列不仅为生物制药生产商提供生产传统和创新治疗药物所需的优质原料,而且帮助他们更快地将产品投放市场并简化监管问题。请相信我们能够为您带来透明的供应链和可靠的全球采购能力,以最佳的法规支持和服务简化您的产品认证。

應用

本产品适用于蛋白质和多肽的体外连接。研究表明转肽酶A可以产生环化蛋白质[1], 并可将多肽与多种取代基偶联,包括聚合物[2]、脂质[3]、荧光团[4]、糖[5]、 微球 [3]、肽核酸 [6]和其它基团。

生化/生理作用

葡萄球菌转肽酶 A 是一种细菌转肽酶,它通过在 LPXTG 识别基序的苏氨酸和甘氨酸之间切割,将蛋白质共价连接到细菌细胞壁,并催化苏氨酸的羧基和细胞壁肽聚糖的氨基之间形成酰胺键 [7]这种化学特性可用于将具有 C 端 LPETGX 基序的蛋白质/肽与其它具有甘氨酸或氨基亚甲基基序的蛋白质或分子进行位点特异性连接[8]

外觀

缓冲水溶液包含:40 mM Tris-HCl,pH 8.0,110 mM NaCl、2.2 mM KCl、8 mM 咪唑、0.04% Tween-20 和 20% 甘油

分析報告

本产品可以在含有 50 mM HEPES (pH=7.4)、150 mM NaCl、5 mM CaCl2、5 mM (Gly)3、25 mM Abz/Dnp 底物和转肽酶 A 的反应缓冲液 (50 μl) 中 在 30°C 下保温 30 分钟进行分析。 荧光强度在 Ex320nm/Em420nm 处测量。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Maximilian W Popp et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(8), 3169-3174 (2011-02-08)
Recombinant protein therapeutics often suffer from short circulating half-life and poor stability, necessitating multiple injections and resulting in limited shelf-life. Conjugation to polyethylene glycol chains (PEG) extends the circulatory half-life of many proteins, but the methods for attachment often lack
John M Antos et al.
Journal of the American Chemical Society, 130(48), 16338-16343 (2008-11-08)
A general chemoenzymatic method for the site-specific attachment of lipids to protein substrates is described. Sortase A is used to append short lipid-modified oligoglycine peptides to the C terminus of protein substrates bearing a five amino acid sortase A recognition
Hongyuan Mao et al.
Journal of the American Chemical Society, 126(9), 2670-2671 (2004-03-05)
Sortase (SrtA), a transpeptidase from Staphylococcus aureus, catalyzes a cell-wall sorting reaction at an LPXTG motif by cleaving between threonine and glycine and subsequently joining the carboxyl group of threonine to an amino group of pentaglycine on the cell wall
Stephan Pritz et al.
The Journal of organic chemistry, 72(10), 3909-3912 (2007-04-17)
Sortase A is a transpeptidase that cleaves at a pentapeptide-motif and subsequently transfers the acyl component to a nucleophile containing N-terminal oligoglycines. We investigate the reaction conditions of the sortase-mediated ligation and demonstrate a useful application by the synthesis of
Peptide-sugar ligation catalyzed by transpeptidase sortase: a facile approach to neoglycoconjugate synthesis.
Sharmishtha Samantaray et al.
Journal of the American Chemical Society, 130(7), 2132-2133 (2008-01-31)

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