SRP0236

PI3 kinase (p110d/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• 同義詞: PI3-kinase p110 subunit delta, PIK3CD, Phosphoinositide-3-kinase, catalytic, delta, ptdIns-3-kinase subunit p110-δ
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥80% (SDS-PAGE)
• 形狀: aqueous solution
• 比活性: ≥1.1 pmol/min-μg
• 分子量: 146 kDa (p110δ); 83.5 kDa (p85α)
• 包裝: pkg of 20 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_005026 (p110delta); XM_043865 (p85alpha)
• UniProt登錄號: O00329
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PIK3CD(5293)

描述

一般說明

Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

安全資訊

• 象形圖: GHS08,GHS07
• 訊號詞: Danger
• 危險聲明: H315,H319,H360D
• 防範說明: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危險分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 1