14-792-M

PI 3-Kinase (p110α(H1047R)/p85α), 10 µg

Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R & untagged, recombinant, human p85α full length.

• 同義詞: Active PI 3-Kinase Protein, PI 3-Kinase p110α/p85α
• 分類程式碼代碼: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 產品線: Upstate^®
• 形狀: liquid
• 比活性: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 分子量: M_w 125 kDa (p110α(H1047R)); M_w 84 kDa (p85α)
• 純化經由: (Ni²⁺/NTA-agarose)
• 物種活性: human
• 製造商／商標名: Upstate^®
• 顏色: colorless
• 溶解度: water: soluble
• 登錄號: U79143
• UniProt登錄號: P26450 (mouse); P27986; P42336 / P27986;P42337 / P26450 (mouse); P42336; P42337 (mouse); U79143
• 應用: life science and biopharma
• 基因資訊: human ... PIK3CA(5290)

描述

一般說明

Research area: Cell Signaling

Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R, and untagged, recombinant, human p85α full length. The H1047R substitution is a somatic mutation in p110α that has been associated with tumours of the colon, stomach, breast, and brain. PI3Ks are categorized into three classes, namely class I, II, and III, based on their structural characteristics, substrate specificities, and the nature of lipid end-products. Class IA PI3Ks are composed of heterodimers that include a p110 catalytic subunit and a p85 regulatory subunit. In mammals, PIK3CA, PIK3CB, and PIK3CD, encode the p110 catalytic isoforms: p110α, p110β, and p110δ, respectively.

生化／生理作用

PI3 kinases play a critical role in various cellular processes such as cell survival, proliferation, differentiation, apoptosis, DNA repair, angiogenesis, cellular metabolism, and motility. PI3Ks can activate the serine/threonine kinase AKT and other downstream effector pathways by transmitting signals from growth factors and cytokines into intracellular messages. Genetic mutation in p110α is observed in breast, pancreatic, colon, liver, and lung cancers.Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and can induce oncogenic transformation.

包裝

Also available in 10 μg size and in bulk

Also available in 250μg size --call for pricing and availability and reference catalog number 14-792M when ordering the 250μg size.

標靶描述

PI3Kα/p85α

外觀

50 mM Tris/HCl pH8.0, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°C）: Not applicable