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Merck
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重要文件

SRP0166

Sigma-Aldrich

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

同義詞:

DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

暫時無法取得訂價和供貨情況

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥90% (SDS-PAGE)

形狀

aqueous solution

分子量

27 kDa (DNMT3L)
59 kDa (DNMT3B)

包裝

pkg of 10 μg

濃度

>0.02 mg/mL

UniProt登錄號

運輸包裝

dry ice

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本產品
BP1031PHR1663Y0000016
Propofol United States Pharmacopeia (USP) Reference Standard

1572503

Propofol

Propofol British Pharmacopoeia (BP) Reference Standard

BP1031

Propofol

Propofol Pharmaceutical Secondary Standard; Certified Reference Material

PHR1663

Propofol

Propofol European Pharmacopoeia (EP) Reference Standard

Y0000016

Propofol

manufacturer/tradename

USP

manufacturer/tradename

BP

manufacturer/tradename

-

manufacturer/tradename

EDQM

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical
pharmaceutical small molecule

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

format

neat

format

neat

format

neat

format

neat

vapor pressure

5.6 mmHg ( 100 °C)

vapor pressure

5.6 mmHg ( 100 °C)

vapor pressure

5.6 mmHg ( 100 °C)

vapor pressure

5.6 mmHg ( 100 °C)

API family

propofol

API family

propofol

API family

propofol

API family

propofol

一般說明

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants.[1] Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene.[2] The gene encoding this protein is localized on human chromosome 20q11.2.[3] DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family.[4] It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.[5]

應用

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

生化/生理作用

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer.[6][1] DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.[7][8]

外觀

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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    ? DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis
    Mark Z. Ma
    EBioMedicine, 2(10), 1340-1350 (2015)
    Association between single-nucleotide polymorphisms of DNMT3L and infertility with azoospermia in Chinese men.
    Huang JX
    Reproductive Biomedicine Online, 24(1), 66-71 (2012)
    DNA methyltransferase 3B -149C/T
    polymorphism and the risk of laryngeal
    squamous cell carcinoma: a case-control
    study
    X. M. Zhang
    Genetics and molecular research : GMR, 14(4), 12866-12871 (2015)
    Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.
    Aapola U
    Genomics, 65(3), 293-298 (2000)
    DNMT3L Modulates Significant and Distinct Flanking Sequence Preference for DNA Methylation by DNMT3A and DNMT3B In Vivo
    Bethany L. Wienholz
    PLoS Genetics, 6(9), e1001106-e1001106 (2010)

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