推薦產品
生物源
human
重組細胞
expressed in baculovirus infected insect cells
籤條
GST tagged (N-terminal)
化驗
≥50% (SDS-PAGE)
形狀
aqueous solution
效力
≥50
分子量
211 kDa
包裝
pkg of 10 μg
製造商/商標名
Sigma-Aldrich
儲存條件
avoid repeated freeze/thaw cycles
濃度
0.12 mg/mL
技術
inhibition assay: suitable
NCBI登錄號
UniProt登錄號
應用
life science and biopharma
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... DNMT1(1786)
一般說明
Research area: Cell cycle
DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases. The DNMT1 gene is mapped to human chromosome 19p13.2.
DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases. The DNMT1 gene is mapped to human chromosome 19p13.2.
The DNMT1 gene is mapped to human chromosome 19p13.2.
應用
DNMT1 (DNA methyltransferase 1) has been used for RNA electrophoretic mobility shift assay (REMSA) to determine the role of extra-coding RNAs (ecRNAs) in controlling neuronal DNA methylation, through interaction with DNA methyltransferases.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
DNMT1 (DNA methyltransferase 1) is the predominant enzyme that is responsible for maintaining the level of DNA methylation. It is responsible for replicating CpG methylation patterns from parent to daughter DNA strands, thus, creating heritable methylation signatures through cell division. It, therefore, maintains epigenetic state of DNA. Abnormalities in the expression of this protein are linked with the progression and prognosis of multiple cancers, such as hepatocellular carcinoma, pancreatic, lung and bladder cancers. Up-regulation of this protein in GC (gastric cancer) with less differentiation, advanced stage and increased rate of mortality. Upregulation of the DNMT1 gene indicates poor prognosis in malignant cancers including renal cell carcinoma, lymphoma, pancreatic and and bladder cancer.
單位定義
One unit is defined as the amount of enzyme required to methylate 1 pmol of DNA substrate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Scientific reports, 6, 38762-38762 (2016-12-09)
Apoptosis inhibitor of macrophage (AIM, encoded by cd5l) is a multi-functional circulating protein that has a beneficial role in the regulation of a broad range of diseases, some of which are ameliorated by AIM administration in mice. In blood, AIM
Differential expression of the epigenetic methylation-related protein DNMT1 by breast cancer molecular subtype and stromal histology.
Journal of Translational Medicine, 14:87 (2016)
Epigenetic regulation of DNA methyltransferases: DNMT1 and DNMT3B in gliomas.
Journal of Neuro-Oncology, 104(2), 483-494 (2011)
Journal of peptide science : an official publication of the European Peptide Society, 25(9), e3200-e3200 (2019-07-17)
Posttranslational modifications of histone proteins, which form nucleosome cores, play an important role in gene regulation. Ubiquitination is one such modification. We previously reported on the synthesis of ubiquitinated histone H3 with an isopeptide mimetic structure. In this report, we
American journal of respiratory cell and molecular biology, 60(3), 323-334 (2018-10-18)
The factors involved in disturbing host homeostasis during sepsis are largely unknown. We sought to determine the immunopathological role of apoptosis inhibitor of macrophage (AIM)/CD5L in sepsis. Here, we show that blockade of AIM led to significantly increased survival after
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