SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• 同義詞: Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• 分類程式碼代碼: 12352204
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in insect cells
• 化驗: ≥90% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: >0.004 units/mg
• 比活性: ≥40 units/μg
• 包裝: pkg of 5 μg
• UniProt登錄號: P10144
• 運輸包裝: wet ice
• 儲存溫度: −70°C
• 基因資訊: human ... GZMB(3002)

描述

一般說明

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

生化／生理作用

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

單位定義

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

外觀

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable