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SRE0001

Sigma-Aldrich

胃蛋白酶 来源于猪胃粘膜

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

同義詞:

胃液素 来源于猪胃, 胃蛋白酶 A

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

品質等級

形狀

lyophilized powder

比活性

≥3200 units/mg protein

分子量

35 kDa

雜質

salt, essentially free

顏色

white to off-white

UniProt登錄號

應用

diagnostic assay manufacturing

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

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相關類別

應用

胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

生化/生理作用

优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

單位定義

一个单位将在 37°C、pH2.0 下每分钟产生 ΔA 280 0.001,以 TCA 可溶产物计,以血红蛋白为底物。(最终体积 = 16 mL。)

分析報告

E1%/280法测定蛋白
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。

其他說明

查看有关 胃蛋白酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

抑制劑

產品號碼
描述
訂價

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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J A Green et al.
Reviews of reproduction, 3(1), 62-69 (1998-03-24)
The pregnancy-associated glycoproteins (PAGs) were first described as placental antigens of cattle that were also present in the blood serum of the mother after implantation. Molecular cloning studies have shown that they are members of the aspartic proteinase gene family
Joseph S Fruton
The Quarterly review of biology, 77(2), 127-147 (2002-07-02)
Studies on gastric digestion during 1820-1840 led to the discovery of pepsin as the agent which, in the presence of stomach acid, causes the dissolution of nutrients such as meat or coagulated egg white. Soon afterward it was shown that
J Tack
Alimentary pharmacology & therapeutics, 22 Suppl 1, 48-54 (2005-07-27)
Gastro-oesophageal reflux disease is defined as the presence of symptoms or lesions that can be attributed to the reflux of gastric contents into the oesophagus. Aspiration and prolonged monitoring studies in humans have shown that reflux of gastric contents is
B I Hirschowitz
The Yale journal of biology and medicine, 72(2-3), 133-143 (2000-04-26)
Esophagitis results from excessive exposure of the esophagus to gastric juice through an ineffective or dysfunctional lower esophageal sphincter mechanism. A possible role of pepsin in damaging the esophageal mucosa with consequent esophagitis may be examined directly by testing pepsin
Autonomic control of secretion of gastric acid and pepsin.
K Bech
Journal of autonomic pharmacology, 9(6), 419-428 (1989-12-01)

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