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SHPHLIBR

Sigma-Aldrich

MISSION® LentiPlex® 人类混合shRNA完整文库

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

分類程式碼代碼:
41105904
NACRES:
NA.51

產品線

MISSION®

濃度

≥5x108 VP/ml (via p24 assay)

技術

capture ELISA: 5 x 108 TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

相關類別

一般說明

MISSION LentiPlex混合文库结合MISSION TRC shRNA集合和Sigma慢病毒生产经验的优势，为研究人员提供覆盖全基因组的RNAi筛选。LentiPlex混合系统对非分裂和原代细胞系的递送和长期基因沉默能力更强，探索潜能无限。方便易用的形式让研究人员可在最少的试剂、时间或固定设备投入下，实现快速的全基因组RNAi筛选。

MISSION LentiPlex Complete Human shRNA Pooled Library（人类shRNA混合完整文库）是通过专有流程生产的覆盖全基因组的慢病毒池（慢病毒载体shRNA文库）。文库共有125,000多个来自TRC集合的shRNA构建体，针对2万多个人类基因。每个文库在放行前都经过部分shRNA检测，确保稳健的文库覆盖。文库为即用型慢病毒，滴度在5 x 108 TU/ml以上（p24蛋白检测法），预分为16个亚池，每个亚池约含6,000-8,000个shRNA构建体。另提供下游目标基因鉴定用的扩增和测序引物。

如需关于样品数据和使用等的更多信息，请访问我们的 LentiPlex技术详情页面。

其他說明

MISSION LentiPlex人类shRNA混合文库由货号SHPH01、SHPH15、SHPH2和SHPHLIBR产品组成。从说明书表1可以了解每个文库的shRNA克隆覆盖、靶基因和亚池的大致数目。

另提供用于下游沉默基因鉴定的扩增和测序引物。

shRNA池反卷积
Sigma的反卷积(deconvolution)服务让研究人员可以轻松发现会影响shRNA混合筛选的基因。

  • 对克隆进行新一代测序可确定shRNA混合样品中特定克隆的准确数目
  • shRNA混合筛选提供全面的、重现性结果
  • 稳健统计和信息丰富的数据

更多信息可联系您当地的Sigma销售代表,或发邮件至MISSION RNAi

法律資訊

慢病毒许可协议
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells.
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology

文章

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FACS Titration of Lentivirus Protocol

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